Patch-clamp fluorometry–based channel counting to determine HCN channel conductance

  title={Patch-clamp fluorometry–based channel counting to determine HCN channel conductance},
  author={Chang Liu and Changan Xie and Khade Grant and Zhuocheng Su and Weihua Gao and Qinglian Liu and Lei Zhou},
  booktitle={The Journal of general physiology},
Counting ion channels on cell membranes is of fundamental importance for the study of channel biophysics. Channel counting has thus far been tackled by classical approaches, such as radioactive labeling of ion channels with blockers, gating current measurements, and nonstationary noise analysis. Here, we develop a counting method based on patch-clamp fluorometry (PCF), which enables simultaneous electrical and optical recordings, and apply it to EGFP-tagged, hyperpolarization-activated and… CONTINUE READING
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