Pasteurella multocida sialic acid aldolase: a promising biocatalyst

@article{Li2008PasteurellaMS,
  title={Pasteurella multocida sialic acid aldolase: a promising biocatalyst},
  author={Yanhong Li and Hai Yu and Hongzhi Cao and Kam Lau and Saddam M. Muthana and Vinod Kumar Tiwari and Bryan Son and Xi Chen},
  journal={Applied Microbiology and Biotechnology},
  year={2008},
  volume={79},
  pages={963-970}
}
Sialic acid aldolases or N-acetylneuraminate lyases (NanAs) catalyze the reversible aldol cleavage of N-acetylneuraminic acid (Neu5Ac) to form pyruvate and N-acetyl-d-mannosamine (ManNAc). A capillary electrophoresis assay was developed to directly characterize the activities of NanAs in both Neu5Ac cleavage and Neu5Ac synthesis directions. The assay was used to obtain the pH profile and the kinetic data of a NanA cloned from Pasteurella multocida P-1059 (PmNanA) and a previously reported… CONTINUE READING

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