Passive mechanical behavior of human neutrophils: effects of colchicine and paclitaxel.

@article{Tsai1998PassiveMB,
  title={Passive mechanical behavior of human neutrophils: effects of colchicine and paclitaxel.},
  author={Mientao A. Tsai and Richard E. Waugh and Peter C. Keng},
  journal={Biophysical journal},
  year={1998},
  volume={74 6},
  pages={3282-91}
}
The role of microtubules in determining the mechanical rigidity of neutrophils was assessed. Neutrophils were treated with colchicine to disrupt microtubules, or with paclitaxel to promote formation of microtubules. Paclitaxel caused an increase in the number of microtubules in the cells as assessed by immunofluorescence, but it had no effect on the presence or organization of actin filaments or on cellular mechanical properties. Colchicine at concentrations <1.0 microM caused disruption of… CONTINUE READING