Passive immunity to yersiniae mediated by anti-recombinant V antigen and protein A-V antigen fusion peptide.

@article{Motin1994PassiveIT,
  title={Passive immunity to yersiniae mediated by anti-recombinant V antigen and protein A-V antigen fusion peptide.},
  author={Vladimir L. Motin and Ryouichi Nakajima and George Smirnov and Robert R. Brubaker},
  journal={Infection and immunity},
  year={1994},
  volume={62 10},
  pages={4192-201}
}
LcrV (V antigen), a known unstable 37.3-kDa monomeric peptide encoded on the ca. 70-kb Lcr plasmid of Yersinia pestis, Yersinia pseudotuberculosis, and Yersinia enterocolitica, has been implicated as a regulator of the low-calcium response, virulence factor, and protective antigen. In this study, lcrV of Y. pestis was cloned into protease-deficient Escherichia coli BL21. The resulting recombinant V antigen underwent marked degradation from the C-terminal end during purification, yielding major… CONTINUE READING