Passenger-Strand Cleavage Facilitates Assembly of siRNA into Ago2-Containing RNAi Enzyme Complexes

@article{Matranga2005PassengerStrandCF,
  title={Passenger-Strand Cleavage Facilitates Assembly of siRNA into Ago2-Containing RNAi Enzyme Complexes},
  author={Christian Matranga and Yukihide Tomari and Chanseok Shin and David P. Bartel and Phillip D. Zamore},
  journal={Cell},
  year={2005},
  volume={123},
  pages={607-620}
}
In the Drosophila and mammalian RNA interference pathways, siRNAs direct the protein Argonaute2 (Ago2) to cleave corresponding mRNA targets, silencing their expression. Ago2 is the catalytic component of the RNAi enzyme complex, RISC. For each siRNA duplex, only one strand, the guide, is assembled into the active RISC; the other strand, the passenger, is destroyed. An ATP-dependent helicase has been proposed first to separate the two siRNA strands, then the resulting single-stranded guide is… CONTINUE READING
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