Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells

@article{Zacharias2002PartitioningOL,
  title={Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells},
  author={D. Zacharias and J. D. Violin and A. Newton and R. Tsien},
  journal={Science},
  year={2002},
  volume={296},
  pages={913 - 916}
}
Many proteins associated with the plasma membrane are known to partition into submicroscopic sphingolipid- and cholesterol-rich domains called lipid rafts, but the determinants dictating this segregation of proteins in the membrane are poorly understood. We suppressed the tendency of Aequoreafluorescent proteins to dimerize and targeted these variants to the plasma membrane using several different types of lipid anchors. Fluorescence resonance energy transfer measurements in living cells… Expand
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