Partitioning behavior and enrichment of proteins with reversed micellar extraction: I. forward extraction of proteins from aqueous to reversed micellar phase.

@article{Nishiki1993PartitioningBA,
  title={Partitioning behavior and enrichment of proteins with reversed micellar extraction: I. forward extraction of proteins from aqueous to reversed micellar phase.},
  author={Tsuneo Nishiki and Ippei Sato and Takao Kataoka and Dai Kato},
  journal={Biotechnology and bioengineering},
  year={1993},
  volume={42 5},
  pages={596-600}
}
Protein extractions using aerosol OT (AOT)-isooctane reverse micelle solutions have been studied to explore the potential for separating and enriching proteins with the reversed micellar extraction. The effects of pH, ionic strength, and different cations of chlorides in a bulk aqueous phase and of AOT concentration in an organic phase on the partitioning of lysozyme and myoglobin and the solubilization of water are presented in detail. The extraction of lysozyme was affected by the… CONTINUE READING