Partition coefficients of substrates and products and solvent selection for biocatalysis under nearly anhydrous conditions

  title={Partition coefficients of substrates and products and solvent selection for biocatalysis under nearly anhydrous conditions},
  author={Z. Yang and Donald A. Robb},
  journal={Biotechnology and Bioengineering},
  • Z. Yang, D. Robb
  • Published 5 March 1994
  • Chemistry
  • Biotechnology and Bioengineering
The effect of solvent on the activity of mushroom tyrosinase toward three substrates was studied at a constant water activity of either 0.74 or 0.86. No simple correlation was observed between enzyme activity and log P, but partition coefficients of substrate (Ps) and product (Pp) gave systematic relations with enzyme activity. When initial reaction rates were considered, there was a bellshaped relationship between enzyme activity and Ps with an optimal Ps for each substrate. This can be… 
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