Particle tracking and inference in fluorescence microscopy

  title={Particle tracking and inference in fluorescence microscopy},
  author={T. H. Thomas},
Observing biophysical phenomena at the nanometer scale with both high spatial and temporal resolution is a challenging feat. Although many techniques, including atomic force microscopy and scanning electron microscopy, have demonstrated subnanometer spatial resolution, most exhibit drawbacks which limit their temporal resolution. On the other hand, light microscopy exhibits poor spatial resolution (typically greater than 100 nm) due to diffraction. The desire to image features below the… CONTINUE READING