Particle-mediated Intravenous Delivery of Antigen mRNA Results in Strong Antigen-specific T-cell Responses Despite the Induction of Type I Interferon

@article{Broos2016ParticlemediatedID,
  title={Particle-mediated Intravenous Delivery of Antigen mRNA Results in Strong Antigen-specific T-cell Responses Despite the Induction of Type I Interferon},
  author={Katrijn Broos and Kevin Van der Jeught and Janik Puttemans and Cleo Goyvaerts and Carlo Heirman and Heleen Dewitte and Rein Verbeke and Ine Lentacker and Kris Thielemans and Karine Breckpot},
  journal={Molecular Therapy. Nucleic Acids},
  year={2016},
  volume={5}
}
Cancer vaccines based on mRNA are extensively studied. The fragile nature of mRNA has instigated research into carriers that can protect it from ribonucleases and as such enable its systemic use. However, carrier-mediated delivery of mRNA has been linked to production of type I interferon (IFN) that was reported to compromise the effectiveness of mRNA vaccines. In this study, we evaluated a cationic lipid for encapsulation of mRNA. The nanometer-sized, negatively charged lipid mRNA particles… 
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