Particle bombardment, a method for gene transfer in marigold

Abstract

Marigold (Tagetes erecta) leaf explants were bombarded with plasmid pBI426 in a low pressure particle inflow gun. Transient expression assays were performed with different pressures and distances. The best transient expression (GUS) level was obtained with explants induced in regeneration medium and bombarded at 10.5 cm and 551.6 kPa. Stable expression… (More)
DOI: 10.1007/s11240-005-9030-x

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