Particle assembly incorporating a VP22-BH3 fusion protein, facilitating intracellular delivery, regulated release, and apoptosis.

@article{Brewis2003ParticleAI,
  title={Particle assembly incorporating a VP22-BH3 fusion protein, facilitating intracellular delivery, regulated release, and apoptosis.},
  author={Neil D Brewis and Anne Phelan and Nadia Normand and Esther Choolun and Peter G O'Hare},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2003},
  volume={7 2},
  pages={262-70}
}
Previously we showed that addition of purified VP22, a structural protein of herpes simplex virus, to short oligonucleotides (ODN) induced the spontaneous assembly of novel particles incorporating both protein and ODN. These particles were not toxic, entered cells, and resided stably in the cytoplasm. Surprisingly the particles could be activated by light in a regulated synchronous manner to release ODN and protein to the cell cytosol and nuclei. Here we construct a fusion protein containing a… CONTINUE READING