Participation of p53 cellular tumour antigen in transformation of normal embryonic cells

@article{Eliyahu1984ParticipationOP,
  title={Participation of p53 cellular tumour antigen in transformation of normal embryonic cells},
  author={Daniel Eliyahu and Avraham Raz and Peter Gruss and David Givol and Moshe Oren},
  journal={Nature},
  year={1984},
  volume={312},
  pages={646-649}
}
The cellular tumour antigen p53 is found at elevated levels in a wide variety of transformed cells (for reviews see refs 1, 2). Very little is yet known about the precise relationship of p53 to malignant transformation. Although the increase in p53 levels could be a secondary by-product of the transformed state, it is equally possible that p53 is actively involved in altering cellular growth properties, especially as it has been implicated in the regulation of normal cell proliferation3–6. We… 
Overproduction of p53 antigen makes established cells highly tumorigenic
TLDR
It is demonstrated here that overproduction of p53 in an established cell line, while not causing gross morphological changes, endows these cells with an overt tumorigenic potential, and the tumorigenics efficiency of such cell lines may be correlated with the extent of p 53 overproduction.
Cooperation between gene encoding p53 tumour antigen and ras in cellular transformation
TLDR
The results indicate that the p53-encoding gene can play a causal role in the conversion of normal fibroblasts into tumorigenic cells, suggesting an important role of p53 in tumorigenesis.
Wild-Type versus Mutant p53
TLDR
Isolation and characterization of the p53 gene followed by early transfection studies indicated that p53 is capable of immortalizing primary rat embryonic fibroblast cells in culture and could cooperate with activated ras oncogene in cellular transformation of primary cells inculture.
Rearrangements of the cellular p53 gene in erythroleukaemic cells transformed by Friend virus
TLDR
It is demonstrated that genomic rearrangements are responsible for p53 gene Jnactivation in these cell lines and that they occur in vivo during the natural progression of Friend virus-induced erythroleukaemia.
Human p53 and human tumours
  • L. Crawford
  • Biology
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1985
TLDR
Observations in culture are not the same as cells in a patient, but these observations do raise questions about the possible involvement of p53 in human tumours.
p53 and human malignancies.
The emerging picture of p53.
Biological Phenotypes of Tumor-Derived Human p53 Mutants
p53 is a cellular protein expressed at low levels in the normal cell (Benchimol et al. 1982; Thomas et al. 1983). p53 was originally discovered as a 53 000 kDa protein that co-immunoprecipitates with
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References

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A single gene and a pseudogene for the cellular tumour antigen p53
TLDR
Evidence is provided for the existence of a single functional gene for murine p53 and a processed pseudogene and the predicted amino acid sequence of murinep53 is presented.
Cooperation between gene encoding p53 tumour antigen and ras in cellular transformation
TLDR
The results indicate that the p53-encoding gene can play a causal role in the conversion of normal fibroblasts into tumorigenic cells, suggesting an important role of p53 in tumorigenesis.
Growth regulation of a cellular tumour antigen, p53, in nontransformed cells
TLDR
An increase in the synthesis and steady-state levels of p53 protein and mRNA prior to DNA synthesis in late G1 is demonstrated and a role for p53 in the progression of cells from a growth-arrested state to an actively dividing state is suggested.
Two distinct mechanisms regulate the levels of a cellular tumor antigen, p53.
TLDR
Evidence is provided that the regulation of p53 expression in cells can occur at the level of p 53 mRNA abundancy or p53 protein stability depending upon the experimental system under study, and that a regulated degradation process controls the turnover of p52 protein.
P53 transformation‐related protein accumulates in the nucleus of transformed fibroblasts in association with the chromatin and is found in the cytoplasm of non‐transformed fibroblasts.
TLDR
The switch from the cytoplasmic localization of p53 in the non‐transformed fibroblasts to a chromatin‐associated accumulation in the transformed cells suggests a possible mechanism by which this protein may function in the transformations.
Variation in antigenic determinants of p53 transformation-related protein obtained from various species.
TLDR
P53 is a single protein that can be immunoprecipitated through different antigenic determinants that vary between species, and RA3-2C2, which recognizes a mouse-specific determinant, binds a site located at a proteolytic digestion fragment of the p53 molecule that differs from that containing PAb122 and PAb421 recognition sites.
Post-translational regulation of the 54K cellular tumor antigen in normal and transformed cells
TLDR
A post-translational regulation of the 54K cellular tumor antigen is demonstrated and it is suggested that this control is mediated by the SV40 large T-antigen.
Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse.
TLDR
The presence of p53 in tumors of no known viral etiology indicates coding by resident cellular genes; this does not exclude endogenous viruses as the source of coding sequences or the possibility that transforming viruses code directly for p53.
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