Participation of dynamin in the biogenesis of cytoplasmic vesicles

@article{Henley1999ParticipationOD,
  title={Participation of dynamin in the biogenesis of cytoplasmic vesicles},
  author={John R. Henley and Hong Cao and Mark A. McNiven},
  journal={The FASEB Journal},
  year={1999},
  volume={13},
  pages={S243 - S247}
}
Dynamin is a 100‐kDa GTPase that has been implicated in endocytosis. To extend our understanding of its cellular functions, we have microinjected specific affinity‐purified anti‐dynamin antibodies into cultured mammalian epithelial cells. Using this approach, dynamin function can be inhibited specifically and rapidly in single cells. Effects of microinjected inhibitory antibodies on distinct endocytic processes and plasmalemmal morphology were then assayed by fluorescence microscopy (FM) and… 
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References

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Dynamin-mediated Internalization of Caveolae
TLDR
Functional in vivo studies as well as ultrastructural and biochemical analyses indicate that dynamin mediates both clathrin-dependent endocytosis and the internalization of caveolae in mammalian cells.
Association of a dynamin-like protein with the Golgi apparatus in mammalian cells
TLDR
This study provides the first morphological and biochemical evidence that a dynamin-like protein associates with the Golgi apparatus in mammalian cells, and suggests that dynamin -related proteins may have multiple cytoplasmic distributions.
Induction of mutant dynamin specifically blocks endocytic coated vesicle formation
TLDR
It is concluded that dynamin is specifically required for endocytic coated vesicle formation, and that its GTP binding and hydrolysis activities are required to form constricted coated pits and, subsequently, for coatedvesicle budding.
Role of dynamin in the formation of transport vesicles from the trans-Golgi network.
TLDR
Data suggest that dynamin participates in the formation of distinct transport vesicles from the trans-Golgi network, and that budding efficiency was restored upon readdition of purified dynamin.
Effects of mutant rat dynamin on endocytosis
TLDR
A specific role is demonstrated for dynamin and for GTP in the initial stages of receptor-mediated endocytosis in cultured mammalian COS-7 cells with both wild-type and mutant dynamin cDNAs.
Biochemical and immunochemical analysis of rat brain dynamin interaction with microtubules and organelles in vivo and in vitro
TLDR
The purified rat brain protein is determined to be dynamin, a putative microtubule-associated motility protein that appears to be specific to neuronal tissue where it is present in both soluble and particulate tissue fractions.
Dynamin is a GTPase stimulated to high levels of activity by microtubules
TLDR
It is reported here that dynamin is a specific GTPase which can be stimulated to very high levels of activity by microtubules, and exhibited microtubule-activated ATPase activity and dynamin-induced bundles showed evidence of ATP-dependent force production.
Activation of dynamin GTPase by acidic phospholipids and endogenous rat brain vesicles.
TLDR
An analysis of the enzymatic and membrane binding properties of dynamin has found that the acidic phospholipids, phosph atidylserine, phosphatidylglycerol, and phosphAtidylinositol, are able to stimulate GTP hydrolysis in a manner similar to activation previously shown with microtubules.
Dynamin at the Neck of Caveolae Mediates Their Budding to Form Transport Vesicles by GTP-driven Fission from the Plasma Membrane of Endothelium
TLDR
Through its ability to oligomerize, dynamin appears to form a structural collar around the neck of caveolae that hydrolyzes GTP to mediate internalization via the fission of Caveolae from the plasma membrane to form free transport vesicles.
Clathrin-independent pinocytosis is induced in cells overexpressing a temperature-sensitive mutant of dynamin
TLDR
It is suggested that a dynamin- and clathrin-independent mechanism contributes to the total constitutive pinocytosis in HeLa cells and that dynts cells rapidly and completely compensate for the loss of clathin- dependent endocytotic by inducing an alternate endocytic pathway.
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