Participation of dynamin in the biogenesis of cytoplasmic vesicles

  title={Participation of dynamin in the biogenesis of cytoplasmic vesicles},
  author={John R. Henley and Hong Cao and Mark A. McNiven},
  journal={The FASEB Journal},
  pages={S243 - S247}
Dynamin is a 100‐kDa GTPase that has been implicated in endocytosis. To extend our understanding of its cellular functions, we have microinjected specific affinity‐purified anti‐dynamin antibodies into cultured mammalian epithelial cells. Using this approach, dynamin function can be inhibited specifically and rapidly in single cells. Effects of microinjected inhibitory antibodies on distinct endocytic processes and plasmalemmal morphology were then assayed by fluorescence microscopy (FM) and… 
Dynamin and its role in membrane fission.
  • J. Hinshaw
  • Biology, Chemistry
    Annual review of cell and developmental biology
  • 2000
Purified dynamin readily self-assembles into rings or spirals, which supports the hypothesis that dynamin wraps around the necks of budding vesicles where it plays a key role in membrane fission.
The Single Dynamin Family Protein in the Primitive Protozoan Giardia lamblia Is Essential for Stage Conversion and Endocytic Transport
Giardial DRP plays a key role in two distinct trafficking pathways and in organelle homeostasis, both essential functions for the proliferation of the parasite in the gut and its transmission to a new host.
Endocytosis of Adeno-Associated Virus Type 5 Leads to Accumulation of Virus Particles in the Golgi Compartment
The data suggest that AAV5 makes use of endocytic routes that have hitherto not been described as pathways for virus entry, and the different binding kinetics suggest different binding properties at apical versus lateral plasma membranes.
Role of Src-induced Dynamin-2 Phosphorylation in Caveolae-mediated Endocytosis in Endothelial Cells*
Src-mediated phosphorylation of dynamin-2 is an essential requirement for scission of caveolae and the resultant transendothelial transport of albumin.
Cell-surface transglutaminase undergoes internalization and lysosomal degradation: an essential role for LRP1
A previously unknown pathway of transglutaminase internalization and degradation that might be crucial for regulation of its adhesive and signaling functions on the cell surface is characterized and a novel functional link between cell-matrix adhesion and endocytosis is revealed.
A Molecular Mechanism to Regulate Lysosome Motility for Lysosome Positioning and Tubulation
It is shown that TRPML1 activity is required to promote Ca2+-dependent centripetal movement of lysosomes towards the perinuclear region (where autophagosomes accumulate) following autophagy induction.
Endocytosis: Receptor-Mediated
Advances in small-molecule probes, structural biology, and genetic methods are beginning to illuminate the complex mechanisms of this process at the molecular level, which can enhance efficiently the cellular uptake of impermeable molecules.
Gβγ Activation of Src Induces Caveolae-mediated Endocytosis in Endothelial Cells*
It is demonstrated that Gβγ activation of Src signals caveolae-mediated endocytosis and transendothelial albumin transport via transcytosis.
Endocytic pathway in mouse cardiac cells.
The results demonstrated the route of the internalized cargo to early endosomes followed or not by its discharge in the late compartments, and confirmed the ability of cardiac muscle cells to ingest large particles, and even internalize whole eukaryotic cells such as the protozoan parasite Trypanosoma cruzi.
A Novel Size-Based Sorting Mechanism of Pinocytic Luminal Cargoes in Microglia
It is reported that a size-dependent selective transport of small soluble contents from the pinosomal lumen into lysosomes is critical for the antigen processing in microglia, and a novel early sorting mechanism forPinosomal luminal contents inmicroglial cells is revealed, which may explain how microglio efficiently process protein antigens and evoke an immune response.


Dynamin-mediated Internalization of Caveolae
Functional in vivo studies as well as ultrastructural and biochemical analyses indicate that dynamin mediates both clathrin-dependent endocytosis and the internalization of caveolae in mammalian cells.
Association of a dynamin-like protein with the Golgi apparatus in mammalian cells
This study provides the first morphological and biochemical evidence that a dynamin-like protein associates with the Golgi apparatus in mammalian cells, and suggests that dynamin -related proteins may have multiple cytoplasmic distributions.
Induction of mutant dynamin specifically blocks endocytic coated vesicle formation
It is concluded that dynamin is specifically required for endocytic coated vesicle formation, and that its GTP binding and hydrolysis activities are required to form constricted coated pits and, subsequently, for coatedvesicle budding.
Role of dynamin in the formation of transport vesicles from the trans-Golgi network.
Data suggest that dynamin participates in the formation of distinct transport vesicles from the trans-Golgi network, and that budding efficiency was restored upon readdition of purified dynamin.
Effects of mutant rat dynamin on endocytosis
A specific role is demonstrated for dynamin and for GTP in the initial stages of receptor-mediated endocytosis in cultured mammalian COS-7 cells with both wild-type and mutant dynamin cDNAs.
Biochemical and immunochemical analysis of rat brain dynamin interaction with microtubules and organelles in vivo and in vitro
The purified rat brain protein is determined to be dynamin, a putative microtubule-associated motility protein that appears to be specific to neuronal tissue where it is present in both soluble and particulate tissue fractions.
Dynamin is a GTPase stimulated to high levels of activity by microtubules
It is reported here that dynamin is a specific GTPase which can be stimulated to very high levels of activity by microtubules, and exhibited microtubule-activated ATPase activity and dynamin-induced bundles showed evidence of ATP-dependent force production.
Activation of dynamin GTPase by acidic phospholipids and endogenous rat brain vesicles.
An analysis of the enzymatic and membrane binding properties of dynamin has found that the acidic phospholipids, phosph atidylserine, phosphatidylglycerol, and phosphAtidylinositol, are able to stimulate GTP hydrolysis in a manner similar to activation previously shown with microtubules.
Dynamin at the Neck of Caveolae Mediates Their Budding to Form Transport Vesicles by GTP-driven Fission from the Plasma Membrane of Endothelium
Through its ability to oligomerize, dynamin appears to form a structural collar around the neck of caveolae that hydrolyzes GTP to mediate internalization via the fission of Caveolae from the plasma membrane to form free transport vesicles.
Clathrin-independent pinocytosis is induced in cells overexpressing a temperature-sensitive mutant of dynamin
It is suggested that a dynamin- and clathrin-independent mechanism contributes to the total constitutive pinocytosis in HeLa cells and that dynts cells rapidly and completely compensate for the loss of clathin- dependent endocytotic by inducing an alternate endocytic pathway.