Participation of GTP-binding protein in the photo-transduction of Paramecium bursaria.

@article{Shinozawa1996ParticipationOG,
  title={Participation of GTP-binding protein in the photo-transduction of Paramecium bursaria.},
  author={Takao Shinozawa and Hisayuki Hashimoto and Jun Fujita and Yasuo Nakaoka},
  journal={Cell structure and function},
  year={1996},
  volume={21 6},
  pages={469-74}
}
Analysis of the flow of light information in Paramecium, especially the participation of GTP-binding protein, was carried out. Injection of GDP-beta-S into Paramecium cell abolished the light-induced inward current. The partially purified rhodopsin-like protein (RLP) that we obtained activated frog rod outer segments (ROS) GTPase. A GTP-binding protein, with Mw 57,000 was detected by immunostaining with anti-rat G alpha rabbit IgG. These results suggest that the light information flows from the… CONTINUE READING