Partial sequencing of the β-glucosidase-encoding gene of yeast strains isolated from musts and wines

  title={Partial sequencing of the β-glucosidase-encoding gene of yeast strains isolated from musts and wines},
  author={Paola Quatrini and Sandra Marineo and Anna Maria Puglia and Cristina Restuccia and Cinzia Caggia and Cinzia Lucia Randazzo and Giovanni Spagna and Riccardo Nunzio Barbagallo and Rosa Palmeri and Paolo Giudici},
  journal={Annals of Microbiology},
The aim of the present work was the identification of the gene encoding for β-glucosidase and its partial sequencing in the strainsPichia anomala AL112,Hanseniaspora uvarum Y8 andSaccharomyces cerevisiae AL41. To this aim degenerated primers, designed on the basis of aminoacid similarities of four known yeast β-glucosidases, have been used in PCR amplifications. An expected fragment of about 200 bp was amplified from all the DNAs, cloned and sequenced. Sequence homology demonstrated for the… CONTINUE READING

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