Partial restoration of the keratocyte phenotype to bovine keratocytes made fibroblastic by serum.

@article{Berryhill2002PartialRO,
  title={Partial restoration of the keratocyte phenotype to bovine keratocytes made fibroblastic by serum.},
  author={Bridgette L Berryhill and Ronald Kader and Bradley P Kane and David E. Birk and Jessie Y. Feng and J. Hassell},
  journal={Investigative ophthalmology & visual science},
  year={2002},
  volume={43 11},
  pages={3416-21}
}
PURPOSE To determine whether keratocytes made fibroblastic in vitro by addition of fetal bovine serum to the medium regain the keratocyte phenotype after culture in serum-free medium. METHODS Collagenase-isolated keratocytes from bovine corneas were plated in DMEM/F-12 containing 1% horse plasma, to allow cell attachment, and then cultured until day 4 in either DMEM/F-12 alone, to retain the keratocyte phenotype, or in DMEM containing 10% fetal bovine serum, to cause the keratocytes to become… CONTINUE READING