Partial reconstitution of a replication-competent retrovirus in helper cells with partial overlaps between vector and helper cell genomes.

@article{Martinez1996PartialRO,
  title={Partial reconstitution of a replication-competent retrovirus in helper cells with partial overlaps between vector and helper cell genomes.},
  author={Isidoro Martinez and Ralph C Dornburg},
  journal={Human gene therapy},
  year={1996},
  volume={7 6},
  pages={705-12}
}
Contamination of retroviral vector preparations with replication-competent retroviruses is a major safety concern in human gene therapy. These can arise by recombination between retroviral vectors and packaging cell sequences. Recently, we constructed new packaging lines, derived from spleen necrosis virus (SNV) that do not contain overlapping regions of homology between these two components (DSH134G and DSH29B cells). These cell lines were tested for the presence of recombination products and… CONTINUE READING
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