Partial purification of thrombopoietin using lectin chromatography.

@article{Hill1986PartialPO,
  title={Partial purification of thrombopoietin using lectin chromatography.},
  author={R. Hill and Jack Levin},
  journal={Experimental hematology},
  year={1986},
  volume={14 8},
  pages={752-9}
}
Thrombopoietin (TPO) was partially purified from the plasma of thrombocytopenic rabbits by ammonium sulfate fractionation and lectin chromatography. Thrombopoietic activity was measured with an in vivo assay that measures platelet production by the incorporation of 75Se-selenomethionine (75SeM) into newly forming platelets in mice. Thrombopoietin was precipitated from rabbit plasma by an ammonium sulfate saturation of 60%-80%. The ammonium sulfate precipitate was further fractionated by… CONTINUE READING

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