Partial purification of gibberellin oxidases from spinach leaves.

  title={Partial purification of gibberellin oxidases from spinach leaves.},
  author={Sarah Jane Gilmour and Anthony B. Bleecker and Jan A. D. Zeevaart},
  journal={Plant physiology},
  volume={85 1},
Four enzyme activities catalyzing the following oxidative steps in the gibberellin (GA) biosynthetic pathway have been extracted from spinach (Spinacia oleracea L.) leaves after exposure to 8 long days: GA(12) --> GA(53) --> GA(44) --> GA(19) --> GA(20). Two of these, GA(53) oxidase and GA(19) oxidase, were separable from the other two, GA(44) oxidase and GA(12) 13-hydroxylase, by anion exchange high performance liquid chromatography (HPLC). Apparent molecular weights of the four enzymes as… CONTINUE READING


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