Partial purification of estrogen-dependent peroxidase of rat uterus and comparison of the properties with those of other animal peroxidases.

@article{Wagai1982PartialPO,
  title={Partial purification of estrogen-dependent peroxidase of rat uterus and comparison of the properties with those of other animal peroxidases.},
  author={Nobuhiko Wagai and T Hosoya},
  journal={Journal of biochemistry},
  year={1982},
  volume={91 6},
  pages={1931-42}
}
An attempt was made to solubilize a peroxidase from the uterine tissue of estrogen-primed rats using various detergents, and the best result was obtained by incubation with 4% cetyltrimethylammonium bromide at 37 degrees C for 60 min. The solubilized material was then dialyzed and subjected to gel filtration on Sephacryl S-200 followed by CM-cellulose chromatography, resulting in a 50-250-fold increase in specific activity over the detergent extract. Some properties of the partially purified… CONTINUE READING