Partial purification of L-ascorbate:ferricytochrome b5 oxidoreductase from rat liver microsomes.


L-Ascorbate:ferricytochrome b5 oxidoreductase from rat liver microsomes was enriched about 750-fold after treatment with a non-ionic detergent with a yield of about 15%. The enriched enzyme still shows four bands in sodium dodecylsulfate polyacrylamide-gel electrophoresis and is a hydrophobic membrane protein. 


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