Partial purification and substrate specificity of a ubiquitin hydrolase from Saccharomyces cerevisiae.

@article{Agell1991PartialPA,
  title={Partial purification and substrate specificity of a ubiquitin hydrolase from Saccharomyces cerevisiae.},
  author={N{\'u}ria Agell and Charlotte Ryan and Milton J Schlesinger},
  journal={The Biochemical journal},
  year={1991},
  volume={273 ( Pt 3)},
  pages={615-20}
}
A ubiquitin hydrolase that removes ubiquitin from a multi-ubiquitinated protein has been purified 600-fold from Saccharomyces cerevisiae. Four different ubiquitin-protein conjugates were assayed as substrates during the purification procedure. Enzymic activities that removed ubiquitin from ubiquitinated histone H2A, a ubiquitin-ubiquitin dimer and a… CONTINUE READING