Partial purification and properties of rat liver glutaminase.

  title={Partial purification and properties of rat liver glutaminase.},
  author={Mulchand Patel and John D. McGivan},
  journal={The Biochemical journal},
  volume={220 2},
The mitochondrial enzyme phosphate-dependent glutaminase was partially purified from rat liver. The enzyme had Mr 290 000 as judged by chromatography on Sephacryl S-300. After sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of the preparation, glutaminase was tentatively identified with a peptide of Mr 73 500. The concentration-dependence on… CONTINUE READING