Partial purification and characterization of two Cladosporium herbarum allergens.

@article{Aukrust1979PartialPA,
  title={Partial purification and characterization of two Cladosporium herbarum allergens.},
  author={L E Aukrust and S M Borch},
  journal={International archives of allergy and applied immunology},
  year={1979},
  volume={60 1},
  pages={68-79}
}
Two important Cladosporium herbarum allergens (Ag-54 and Ag-32) were partly purified by means of diafiltration, gel filtration and isoelectric focusing. Modifications of quantitative immunological techniques, including autoradiography, were used as assay systems for allergenic activity and to provide a basis for evaluation of the immunological purity of the allergens. Ag-54 appeared to have a molecular weight of approximately 25,000 daltons and an isoelectric point of 5.0. The molecular weight… CONTINUE READING

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