Partial purification and characterization of pyruvate, orthophosphate dikinase from Rhodospirillum rubrum.

@article{Ernst1986PartialPA,
  title={Partial purification and characterization of pyruvate, orthophosphate dikinase from Rhodospirillum rubrum.},
  author={Sjef M P G Ernst and Raymond J A Budde and Raymond Chollet},
  journal={Journal of bacteriology},
  year={1986},
  volume={165 2},
  pages={
          483-8
        }
}
We confirmed an earlier report (B. B. Buchanan, J. Bacteriol. 119:1066-1068, 1974) that the nonsulfur purple photosynthetic bacterium Rhodospirillum rubrum contains pyruvate, orthophosphate dikinase (EC 2.7.9.1) activity that is absolutely dependent upon all three substrates by performing enzyme assays in both the forward (phosphoenolpyruvate formation) and reverse (ATP formation) directions. Of the various carbon sources tested, photoheterotrophic growth on DL-lactate plus bicarbonate proved… CONTINUE READING