Partial purification and characterization of a triglyceride lipase from cod (Gadus morhua)

@inproceedings{Gjellesvik1989PartialPA,
  title={Partial purification and characterization of a triglyceride lipase from cod (Gadus morhua)},
  author={Dag Rune Gjellesvik and Arnt Johan Raae and Bernt Th. Walther},
  year={1989}
}
Abstract Extracts from defatted cod poloric ceaca were used as enzyme source in order to purify and study digestive triglyceride lipase in the cod. By combining ammonium sulphate fractionation, DEAE-cellulose chromatography and gel filtration, a cod triacyl glycerol lipase was purified 200-fold from cod pyloric caeca. The cod lipase showed an absolute requirement for bile salts on olive oil hydrolysis. When tributyrine was used as substrate, the bile salt dependence was less pronounced. The… CONTINUE READING

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