Partial characterization of rat heart cytosolic phospholipase A1 and demonstration of essential sulfhydryl groups.


The cytosol of rat heart has been previously shown to contain phospholipase A activity in substantial amounts. This paper describes the isolation and partial purification of rat heart cytosolic phospholipase A. After homogenization of rat heart followed by centrifugation to remove membraneous material, the phospholipase A activity was isolated by ammonium… (More)


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