Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata)

  title={Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata)},
  author={H{\'e}ctor Nolasco and Francisco J Moyano-L{\'o}pez and Fernando Vega-Villasante},
  journal={Fish Physiology and Biochemistry},
In the present study, we report the isolation and characterization of seabream Sparus aurata pyloric caeca-duodenal lipase. Optimum activity was found at pH 8.5 and salinity of 50 mM NaCl. Lipase activity was sensitive to divalent ions, and extreme pH values (4, 5, and 12), being more stable at alkaline than acid pH. Optimum temperature was found at 50°C, but lipase was stable at temperatures below 40°C. Lipase has a bile salt sodium taurocholate requirement for increased activity. Gradient… CONTINUE READING
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