Partial cDNA cloning of the 230-kD mouse bullous pemphigoid antigen by use of a human monoclonal anti-basement membrane zone antibody.

@article{Amagai1990PartialCC,
  title={Partial cDNA cloning of the 230-kD mouse bullous pemphigoid antigen by use of a human monoclonal anti-basement membrane zone antibody.},
  author={Masayuki Amagai and Takashi Hashimoto and Shigeru Tajima and Y. Inokuchi and Noriaki Shimizu and Makiko Saito and Keizaburo Miki and Takeji Nishikawa},
  journal={The Journal of investigative dermatology},
  year={1990},
  volume={95 3},
  pages={252-9}
}
A cDNA clone with the coding sequence for the 230-kD bullous pemphioid (BP) antigen was isolated from a mouse cDNA expression library by using an anti-basement membrane zone human monoclonal antibody (MoAb-5E). The lambda gt11 cDNA expression library was constructed from poly(A)+RNA from the mouse epidermal cell line, Pam cells, by random priming. 1.5 X 10(5) recombinant clones were screened by immunostaining with MoAb-5E and one positive clone (BPM1) was obtained. All of the ten BP sera but… CONTINUE READING

Similar Papers

Loading similar papers…