Partial amino acid sequence of an L-amino acid oxidase from the cyanobacterium Synechococcus PCC6301, cloning and DNA sequence analysis of the aoxA gene.

@article{Bockholt1995PartialAA,
  title={Partial amino acid sequence of an L-amino acid oxidase from the cyanobacterium Synechococcus PCC6301, cloning and DNA sequence analysis of the aoxA gene.},
  author={R Bockholt and Bernd Masepohl and Volker Kruft and Brigitte Wittmann-Liebold and Elfriede K. Pistorius},
  journal={Biochimica et biophysica acta},
  year={1995},
  volume={1264 3},
  pages={289-93}
}
A novel type of L-amino acid oxidase from Synechococcus PCC6301 was purified and subjected to amino acid sequence analysis. Since the N-terminus of the L-amino acid oxidase protein was not accessible for Edman degradation, the protein was partially hydrolysed and a contiguous sequence of 17 amino acid residues was obtained from an endogenous peptide fragment. Based on the partial peptide sequence two oligonucleotides were designed, which were used as probes in Southern hybridization experiments… CONTINUE READING

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