Partial Purification of the NADH-Nitrate Reductase Complex from Chlorella pyrenoidosa.

Abstract

The nitrate reductase complex from Chlorella pyrenoidosa has been purified by a procedure which includes as main steps, ammonium sulfate fractionation, polyethylene glycol treatment, and DEAE-cellulose chromatography. The Michaelis constants for NADH, FAD, and NO(3) (-) in the NADH-nitrate reductase assay are 10 mum, 2.6 mum, and 0.23 mm, respectively. Heat… (More)

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@article{Schloemer1973PartialPO, title={Partial Purification of the NADH-Nitrate Reductase Complex from Chlorella pyrenoidosa.}, author={R H Schloemer and Reginald H. Garrett}, journal={Plant physiology}, year={1973}, volume={51 3}, pages={591-3} }