Partial Purification of Soluble 5′-nucleotidase from Rabbit Heart: 39


Cytosolic 5′-nucleotidase from rabbit heart has been purified approximately 40-fold to a specific activity of 0.6 μmol/mg protein/min. At this stage of purification, the enzyme is free of other phosphatases and is stable to repeated cycles of freezing and thawing over a 3 months period. Soluble 5′-nucleotidase has an absolute requirement for divalent metal… (More)
DOI: 10.1203/00006450-198507000-00059


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