Partial Purification and Characterization of a Hepatocyte Growth Factor Produced by Rat Hepatocellular Carcinoma Cells 1

@inproceedings{Luetteke2006PartialPA,
  title={Partial Purification and Characterization of a Hepatocyte Growth Factor Produced by Rat Hepatocellular Carcinoma Cells 1},
  author={Noreen C. Luetteke and George K Michalopoulos},
  year={2006}
}
Serum-free medium conditioned by confluent cultures of JM1 or JM2 rat hepatocellular carcinoma cells stimulated DMA syn thesis in primary cultures of adult rat hepatocytes in a dosedependent, saturable manner and in the absence of epidermal growth factor. The hepatotrophic activity was nondialyzable in Mr 50,000 cutoff membranes, heat (60°C)and acid stable, and sensitive to trypsin and dithiothreitol treatment. Gel filtration of concentrated JM1 or JM2 conditioned medium on Sephadex G100… CONTINUE READING