Parthenogenetic development of activated in vitro matured bovine oocytes.

  title={Parthenogenetic development of activated in vitro matured bovine oocytes.},
  author={Radek Prochazka and R Durnford and P S Fiser and Gill Marcus},
  volume={39 5},
Bovine oocytes matured in vitro for 26 hours were electrically stimulated 1) by a single pulse (Treatment A); 2) by 3 pulses 30 minutes apart (Treatment B); 3) by a single pulse followed by 5 minutes of incubation in the stimulation medium (Treatment C); or 4) by a single pulse at 27 hours of maturation (Treatment D). The oocytes were then cultured for up to 8 days to assess parthenogenetic activation and development. Each electrical stimulation consisted of a 60-mus square wave pulse of 2.5 or… CONTINUE READING

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