Parenchymal cells proliferate and differentiate in an organotypic slice culture of the neonatal liver

Abstract

 We applied organotypic slice culture of neonatal mouse liver tissues to maintain the parenchymal cells in ontogenesis and to investigate their proliferation and differentiation. Cultured tissue spread gradually over 3 weeks. Small basophilic cells formed several layers in the center of the cultured tissues, and a monolayer of polygonal cells was seen at… (More)
DOI: 10.1007/s004290050231

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