Parathyroid hormone stimulates ecto-5'-nucleotidase activity in renal epithelial cells: role of protein kinase-C.

@article{Siegfried1995ParathyroidHS,
  title={Parathyroid hormone stimulates ecto-5'-nucleotidase activity in renal epithelial cells: role of protein kinase-C.},
  author={G Siegfried and François Vrtovsnik and Dominique Pri{\'e} and Claude Amiel and G{\'e}rard Friedlander},
  journal={Endocrinology},
  year={1995},
  volume={136 3},
  pages={1267-75}
}
PTH-induced phosphaturia is exerted in part by cAMP added to the renal tubular lumen under the influence of the hormone. Modulation of renal phosphate transport by luminal cAMP requires degradation of the nucleotide into adenosine by brush-border membrane ectoenzymes, among them ecto-5'-nucleotidase (5'-NU). Hormonal modulation of 5'-NU activity was evaluated in cultured opossum kidney cells. PTH (1-100 nM) stimulated 5'-NU in a time-, concentration-, and protein synthesis-dependent manner. The… CONTINUE READING