The 2013 Nobel Prize in Physiology or Medicine recognized pioneering research on secretion of proteins outside the cell and the delivery of selected cargo to specific intracellular compartments via secretory vesicles. This seminal work, performed in yeast and mammalian cells, defined multiple vital roles vesicles play in cellular physiology. Recent studies have revealed the critical role of vesicles in intercellular communication. Here, we provide an overview of eukaryotic parasite-associated extracellular vesicles (EVs). Their contribution to host–pathogen interactions and possible use in vaccine design and disease diagnosis is discussed.