Parameters affecting frequency of CRISPR/Cas9 mediated targeted mutagenesis in rice

@article{Mikami2015ParametersAF,
  title={Parameters affecting frequency of CRISPR/Cas9 mediated targeted mutagenesis in rice},
  author={Masafumi Mikami and Seiichi Toki and Masaki Endo},
  journal={Plant Cell Reports},
  year={2015},
  volume={34},
  pages={1807-1815}
}
Frequency of CRISPR/Cas9-mediated targeted mutagenesis varies depending on Cas9 expression level and culture period of rice callus. Recent reports have demonstrated that the CRISPR/Cas9 system can function as a sequence-specific nuclease in various plant species. Induction of mutation in proliferating tissue during embryogenesis or in germline cells is a practical means of generating heritable mutations. In the case of plant species in which cultured cells are used for transformation, non… CONTINUE READING