Paramagnetic proteoliposomes containing a pure, native, and oriented seven-transmembrane segment protein, CCR5

@article{Mirzabekov2000ParamagneticPC,
  title={Paramagnetic proteoliposomes containing a pure, native, and oriented seven-transmembrane segment protein, CCR5},
  author={Tajib A. Mirzabekov and Harry Kontos and Michael Farzan and Wayne A. Marasco and Joseph Sodroski},
  journal={Nature Biotechnology},
  year={2000},
  volume={18},
  pages={649-654}
}
Seven-transmembrane segment, G protein-coupled receptors play central roles in a wide range of biological processes, but their characterization has been hindered by the difficulty of obtaining homogeneous preparations of native protein. We have created paramagnetic proteoliposomes containing pure and oriented CCR5, a seven-transmembrane segment protein that serves as the principal coreceptor for human immunodeficiency virus (HIV-1). The CCR5 proteoliposomes bind the HIV-1 gp120 envelope… CONTINUE READING