Parallel tagged amplicon sequencing reveals major lineages and phylogenetic structure in the North American tiger salamander (Ambystoma tigrinum) species complex.

@article{ONeill2013ParallelTA,
  title={Parallel tagged amplicon sequencing reveals major lineages and phylogenetic structure in the North American tiger salamander (Ambystoma tigrinum) species complex.},
  author={Eric M. O'Neill and Rachel Schwartz and Charles T. Bullock and Joshua S M Williams and Howard Bradley Shaffer and X. Aguilar-Miguel and Gabriela Parra-Olea and David W. Weisrock},
  journal={Molecular ecology},
  year={2013},
  volume={22 1},
  pages={111-29}
}
Modern analytical methods for population genetics and phylogenetics are expected to provide more accurate results when data from multiple genome-wide loci are analysed. We present the results of an initial application of parallel tagged sequencing (PTS) on a next-generation platform to sequence thousands of barcoded PCR amplicons generated from 95 nuclear loci and 93 individuals sampled across the range of the tiger salamander (Ambystoma tigrinum) species complex. To manage the bioinformatic… CONTINUE READING
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