Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae.

@article{Deutschbauer2002ParallelPA,
  title={Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae.},
  author={Adam M. Deutschbauer and Roy M. Williams and Angela M Chu and Ronald W. Davis},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 24},
  pages={15530-5}
}
We have quantitatively monitored the sporulation and germination efficiencies of approximately 4,200 yeast deletion strains in parallel by using a molecular bar coding strategy. In a single study, we doubled the number of genes functionally implicated in sporulation to approximately 400, identifying both positive and negative regulators. Our set of 261 sporulation-deficient genes illustrates the importance of autophagy, carbon utilization, and transcriptional machinery during sporulation. These… CONTINUE READING