Parallel in Vivo and in Vitro Selection Using Phage Display Identifies Protease-dependent Tumor-targeting Peptides*

@inproceedings{Whitney2010ParallelIV,
  title={Parallel in Vivo and in Vitro Selection Using Phage Display Identifies Protease-dependent Tumor-targeting Peptides*},
  author={Mike Whitney and Jessica L. Crisp and Emilia S. Olson and Todd A. Aguilera and Larry A Gross and Lesley G. Ellies and Roger Tsien},
  booktitle={The Journal of biological chemistry},
  year={2010}
}
We recently developed activatable cell-penetrating peptides (ACPPs) that target contrast agents to in vivo sites of matrix metalloproteinase activity, such as tumors. Here we use parallel in vivo and in vitro selection with phage display to identify novel tumor-homing ACPPs with no bias for primary sequence or target protease. Specifically, phage displaying a library of ACPPs were either injected into tumor-bearing mice, followed by isolation of cleaved phage from dissected tumor, or isolated… CONTINUE READING