Paraformaldehyde effect on ruthenium red and lysine preservation and staining of the staphylococcal glycocalyx.

@article{Fassel1997ParaformaldehydeEO,
  title={Paraformaldehyde effect on ruthenium red and lysine preservation and staining of the staphylococcal glycocalyx.},
  author={T A Fassel and Paul Edward Mozdziak and James Sanger and Charles E. Edmiston},
  journal={Microscopy research and technique},
  year={1997},
  volume={36 5},
  pages={422-7}
}
The utility of lysine in glutaraldehyde-ruthenium red fixatives for the preservation and/or staining of the fibrous staphylococci glycocalyx was improved by inclusion of paraformaldehyde. Short, 20 min prefixation times for paraformaldehyde-glutaraldehyde fixatives containing lysine, with or without ruthenium red, were compared to an extended overnight fixation. Samples were often lost in fixatives that did not contain paraformaldehyde at extended fixation times hampering the effective use of… CONTINUE READING