Paraffin sections of 70-100 μm: a novel technique and its benefits for studying the nervous system.

@article{Feldengut2013ParaffinSO,
  title={Paraffin sections of 70-100 μm: a novel technique and its benefits for studying the nervous system.},
  author={Simone Feldengut and Kelly del Tredici and Heiko Braak},
  journal={Journal of neuroscience methods},
  year={2013},
  volume={215 2},
  pages={241-4}
}
This technical communication provides a protocol for processing paraffin-embedded tissue blocks from the nervous system sectioned at 70-100 μm. Staining is performed on free-floating sections. Unlike 4-15 μm thick paraffin sections, this technique permits (1) the accurate recognition of specific cell types and cytoarchitectonic units (subcortical nuclei and… CONTINUE READING