Paraffin oil as a “methane vector” for rapid and high cell density cultivation of Methylosinus trichosporium OB3b

Abstract

Slow growth and relatively low cell densities of methanotrophs have limited their uses in industrial applications. In this study, a novel method for rapid cultivation of Methylosinus trichosporium OB3b was studied by adding a water-immiscible organic solvent in the medium. Paraffin oil was the most effective at enhancing cell growth and final cell density. This is at least partially due to the increase of methane gas transfer between gas and medium phases since methane solubility is higher in paraffin than in water/nitrate minimal salt medium. During cultivation with paraffin oil at 5% (v/v) in the medium, M. trichosporium OB3b cells also showed higher concentrations of the intermediary metabolites, such as formic acid and pyruvic acid, and consumed more methane compared with the control. Paraffin as methane vector to improve methanotroph growth was further studied in a 5-L fermentor at three concentrations (i.e., 2.5%, 5%, and 10%). Cell density reached about 14 g dry weight per liter with 5% paraffin, around seven times higher than that of the control (without paraffin). Cells cultivated with paraffin tended to accumulate around the interface between oil droplets and the water phase and could exist in oil phase in the case of 10% (v/v) paraffin. These results indicated that paraffin could enhance methanotroph growth, which is potentially useful in cultivation of methanotrophs in large scale in industry.

DOI: 10.1007/s00253-009-1866-2

8 Figures and Tables

05010020102011201220132014201520162017
Citations per Year

80 Citations

Semantic Scholar estimates that this publication has 80 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Han2009ParaffinOA, title={Paraffin oil as a “methane vector” for rapid and high cell density cultivation of Methylosinus trichosporium OB3b}, author={Bing Han and Tao Su and Hao Wu and Zhongxuan Gou and Xin-Hui Xing and Hao Jiang and Ying Chen and Xin Li and John Colin Murrell}, journal={Applied Microbiology and Biotechnology}, year={2009}, volume={83}, pages={669-677} }