Paraffin-embedded tissue as a source of RNA for gene expression analysis in oral malignancy.

@article{Cairns1997ParaffinembeddedTA,
  title={Paraffin-embedded tissue as a source of RNA for gene expression analysis in oral malignancy.},
  author={M. Cairns and S. Church and P. Johnston and K. Phenix and J. Marley},
  journal={Oral diseases},
  year={1997},
  volume={3 3},
  pages={
          157-61
        }
}
OBJECTIVE To assess the feasibility of using archival oral mucosal tissue to examine gene expression at the ribonucleic acid (RNA) level. MATERIALS AND METHODS We describe the isolation of RNA from 8 microns sections of formalin-fixed paraffin-embedded oral mucosal tissue. RNA was reverse transcribed and three candidate genes amplified by polymerase chain reaction (PCR). The ribosomal protein S14 gene is a housekeeping gene which has been used as an internal standard in several quantitative… Expand
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The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa.
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References

SHOWING 1-10 OF 16 REFERENCES
Amplification of human beta-actin gene by the reverse transcriptase-polymerase chain reaction: implications for assessment of RNA from formalin-fixed, paraffin-embedded material.
RT-PCR detection of mRNA recovered from archival glass slide smears.
Primary structure of human ribosomal protein S14 and the gene that encodes it.
...
1
2
...