Paracrine IL-2 Is Required for Optimal Type 2 Effector Cytokine Production.

Abstract

IL-2 is a pleiotropic cytokine that promotes the differentiation of Th cell subsets, including Th1, Th2, and Th9 cells, but it impairs the development of Th17 and T follicular helper cells. Although IL-2 is produced by all polarized Th subsets to some level, how it impacts cytokine production when effector T cells are restimulated is unknown. We show in this article that Golgi transport inhibitors (GTIs) blocked IL-9 production. Mechanistically, GTIs blocked secretion of IL-2 that normally feeds back in a paracrine manner to promote STAT5 activation and IL-9 production. IL-2 feedback had no effect on Th1- or Th17-signature cytokine production, but it promoted Th2- and Th9-associated cytokine expression. These data suggest that the use of GTIs results in an underestimation of the presence of type 2 cytokine-secreting cells and highlight IL-2 as a critical component in optimal cytokine production by Th2 and Th9 cells in vitro and in vivo.

DOI: 10.4049/jimmunol.1601792

Cite this paper

@article{Olson2017ParacrineII, title={Paracrine IL-2 Is Required for Optimal Type 2 Effector Cytokine Production.}, author={Matthew R Olson and Benjamin J Ulrich and Sarah A Hummel and Ibrahim Khan and Brice Meuris and Yesesri Cherukuri and Alexander L Dent and Sarath Chandra Janga and Mark H Kaplan}, journal={Journal of immunology}, year={2017}, volume={198 11}, pages={4352-4359} }