Paracoccidioides brasiliensis presents two different cDNAs encoding homologues of the fructose 1,6-biphosphate aldolase: protein isolation, cloning of the cDNAs and genes, structural, phylogenetic, and expression analysis.

@article{Carneiro2005ParacoccidioidesBP,
  title={Paracoccidioides brasiliensis presents two different cDNAs encoding homologues of the fructose 1,6-biphosphate aldolase: protein isolation, cloning of the cDNAs and genes, structural, phylogenetic, and expression analysis.},
  author={L{\'i}lian Carla Carneiro and Fabr{\'i}cia Paula de Faria and Maria Sueli Soares Felipe and Maristela Pereira and C{\'e}lia Maria de Almeida Soares},
  journal={Fungal genetics and biology : FG & B},
  year={2005},
  volume={42 1},
  pages={51-60}
}
A proteomic approach was used to identify a 39 kDa antigen of Paracoccidioides brasiliensis. Amino acid sequences of the N-terminal and of endoproteinase Lys-C digested peptides revealed the protein to be a fructose 1,6-biphosphate aldolase (FBA) Class II of P. brasiliensis. Two cDNA homologues, Pbfba1 and Pbfba2, were cloned and characterized. Pbfba1 encoded a predicted polypeptide of 360 amino acids that was highly homologous in the primary structure to the same enzyme from fungi and bacteria… CONTINUE READING