Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull-meninges connections

@inproceedings{Cai2018PanopticIO,
  title={Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull-meninges connections},
  author={Ruiyao Cai and Chenchen Pan and Alireza Ghasemigharagoz and Mihail Ivilinov Todorov and Benjamin F{\"o}rstera and Shan Zhao and Harsharan S Bhatia and Arnaldo Parra-Damas and Leander Mrowka and Delphine Theodorou and Markus Rempfler and Anna L R Xavier and Benjamin T Kress and Corinne Benakis and Hanno Steinke and Sabine Liebscher and Ingo Bechmann and Arthur Liesz and Bj{\"o}rn H. Menze and Martin Kerschensteiner and Maiken Nedergaard and Ali Ert{\"u}rk},
  booktitle={Nature Neuroscience},
  year={2018}
}
Analysis of entire transparent rodent bodies after clearing could provide holistic biological information in health and disease, but reliable imaging and quantification of fluorescent protein signals deep inside the tissues has remained a challenge. Here, we developed vDISCO, a pressure-driven, nanobody-based whole-body immunolabeling technology to enhance the signal of fluorescent proteins by up to two orders of magnitude. This allowed us to image and quantify subcellular details through bones… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 13 CITATIONS

Tissue Clearing and Its Application to Bone and Dental Tissues

  • Journal of dental research
  • 2019
VIEW 6 EXCERPTS
CITES METHODS & BACKGROUND
HIGHLY INFLUENCED

Pericytes in Early Vascular Development

VIEW 5 EXCERPTS
CITES BACKGROUND & RESULTS
HIGHLY INFLUENCED

References

Publications referenced by this paper.